Macaranga bancana is one of the Indonesian medicinal plants that is empirically used to treat infectious disease. The aim of this study was to evaluate anti-bacterial and anti-fungal  activities of  M. bancana against human pathogenic microbes, i.e., Escherichia coli, Staphylacoccus aureus, and Candida albicans. The dried leaves of M. bancana were extracted using cold extraction method with n-hexane, dichloromethane, ethylacetate, methanol, and ethanol solvents. The evaluation of anti-microbial activity from these extracts at the concentration of  1000 µg/ml was conducted using microdilution method. Amoxicillin, cephadroxil, and ketoconazole were used as positive controls. All extracts showed 100% inhibitory activity against S. aureus, with the exception of ethanol extract which was 72.8%. Nevertheless, methanol and ethanol extracts showed 100%  inhibitory activity against E. coli, while the lowest activity was shown by n-hexane extract ( 45.2%). All extracts exhibited 100% inhibitory activity against C. albicans.  In conclusion, leaves extract of M. bancana is a potential source of anti-bacterial and anti-fungal agents.  Keywords:  anti-bacterial, anti-fungal, M. bancana, microdilution

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