Soybean is an important commodity after rice and corn. The attack of plant pest organisms in cultivation decreased soybean productivity. Anthracnose is a disease that can reduce soybean production to 95%. The use of excessive synthetic chemical fungicides causes resistance to plant disturbing organisms and leaves chemical residues. The solution to overcome this problem is pay attention using types of fungicide active ingredients and span application time. This study aims to determine the effect of several types of fungicides and span application time on the development of anthracnose and yields. This research was carried out from February to April 2018 on Agroteknopark Jubung land of Jember University. This study used a Factorial Randomized Group Design model with 9 combinations of treatments and 4 replications. The first factor of the type of fungicide consisted of three levels, namely Triadimefon, T. harzianum and betel leaf. The second factor is the span application time of 3.5 and 7 days. The research data were analyzed using variance analysis, if there were significant differences Duncan's Multiple Range Test (DMRT) test was carried out at the level of 5%. Observation parameters included the disease incidence, diseases severity, rate of infection, and weight of pods. The results of the study showed that the combination treatment of PT3 (vegetable fungicide with betel leaves with span application time of 7 days) was the most efficient than the other treatments and gave the results of the rate of disease infection (0.84 units per day).Keywords: anthracnose, fungicide, infection rate, soybeans

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