AbstrakPenambatan nitrogen oleh bakteri rhizosfer dapat dimanfaatkan untuk menyiasati dampak salinitas pada tanah sawah pesisir. Kemampuan tersebut disebabkan oleh aktivitas nitrogenase yang disandikan gen nifH pada komponen II. Penelitian ini bertujuan menganalisis aktivitas nitrogenase pada kondisi salin dan mengidentifikasi gen nifH. Sebanyak 50 isolat bakteri rhizosfer asal tanah sawah pesisir daerah Eretan dan Patimban, Jawa Barat telah dianalisis. Lima isolat yang menunjukkan aktivitas nitrogenase pada kondisi salin adalah Er B1 3, Er B1 4, Er B1 9, Er B2 10, dan Ptb B1 4. Gen nifH kelima sampel diidentifikasi menggunakan PCR menghasilkan amplikon berukuran ~360 bp. Aktivitas nitrogenase tertinggi berdasarkan Analisis Reduksi Asetilen (ARA) diperoleh pada isolat Er B2 10 yang memiliki kekerabatan terdekat dengan bakteri Providencia sp. Hasil yang diperoleh membuktikan bahwa beberapa bakteri asal sawah pesisir dapat menambat nitrogen pada kondisi salin.AbstractThe ability of nitrogen fixation by rhizosphere bacteria could be used to decrease salinity impact in coastal paddy field, due to nitrogenase capability, encoded by a nifH gene in component II. The objectives of this research are to analyze nitrogenase activity in saline condition and identify the presense of the nifH gene. A total of 50 isolates of the rhizosphere bacteria coastal from wetland areas of Eretan and Patimban, West Java, has been isolated and being analyzed. Among them, five isolates i.e. Er B1 3, ER B1 4, Er B1 9, Er B2 10 and Ptb B1 4, showed the nitrogenase activity under saline condition. The polymerase chain reaction (PCR) of the nifH gene from those five samples resulted in the amplicon size of ~360 bp. The highest activity of nitrogenase assessed by acetylene reduction assay (ARA) was shown by Er B2 10 which closely related to bacteria of Providencia sp. The obtained result showed that several bacteria from coastal paddy field were able to conduct nitrogen fixation under saline stress.
| Published by | Universitas Islam Negeri Syarif Hidayatullah Jakarta |
| Journal Name | AL KAUNIYAH |
| Contact Phone | - |
| Contact Name | Dr. Nani Radiastuti |
| Contact Email | n_radiastuti@yahoo.com |
| Location | Kota tangerang selatan, Banten INDONESIA |
| Website | kauniyah| http://journal.uinjkt.ac.id/index.php/kauniyah| |
| ISSN | ISSN : 19783736, EISSN : 25026720, DOI : 10.15408/kauniyah, |
| Core Subject | Science, |
| Meta Subject | Biochemistry, Genetics & Molecular Biology, Materials Science & Nanotechnology, |
| Meta Desc | Al-Kauniyah: Jurnal Biologi (p-ISSN: 1978-3736, e-ISSN: 2502-6720) is an Open Access Journal published by Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islamic University Jakarta, and established since 2007. Since 2016 Al-Kauniyah has established a collaboration with the Association of Lecturer in Biology and Biology Education throughout the State Islamic Higher University (PTKIN) in Indonesia. Until 2015, Al-Kauniyah covered environmental biology solely, but since 2016 the journal has been extended to cover the entire field of biological science (bioscience). By publishing biannually, on April and October, Al-Kauniyah is intended to communicate original researches and current issues on the subject of biology. Since volume 9 issue 1 April 2016, Al-Kauniyah had been changes the layout. This journal warmly welcomes contributions from scholars of related disciplines. Manuscripts can be submitted to AL-KAUNIYAH |
| Penulis | Susilowati, Dwi Ningsih , Setyowati, Mamik |
| Publisher Article | Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami |
| Subtitle Article | Al-Kauniyah: Jurnal Biologi Vol 9, No 2 (2016): Al-Kauniyah Jurnal Biologi |
| Scholar Google | http://scholar.google.com/scholar?q=%2Bintitle%3A&… |
| View Article | http://journal.uinjkt.ac.id/in… |
| DOI | https://doi.org/10.15408/kauniyah.v… |
| DOI Number | DOI: 10.15408/kauniyah.v9i2.4036 |
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