A mesophile bacteria isolated from intestinal systems of local-soil termite, produced an extracellular β-endoxylanase upon growth on xylan. Optimum production of the enzyme was found in media containing oatspelt xylan at 37oC after sixth hours. The activities of its β-endoxylanase on oat-spelt xylan was investigated. It had an optimum pH and temperature, 5.0 and 40o C, respectively. However, pH stability occurred between 5.08.0. The enzyme was stable at 40o C for four hours and possessed a half life of four hours. β-endoxylanase had an apparent molecular mass of 45.000 to 66.200 Dalton as determined by SDS-PAGE. Analysis of zymogram using SDS-Xylan-PAGE indicated that enzymes could degrade oat-spelt xylan as substrates.

Published by Universitas Jember
Journal Name Jurnal ILMU DASAR
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Location Kab. jember, Jawa timur INDONESIA
Website JID| https://jurnal.unej.ac.id/index.php/JID|
ISSN ISSN : 14115735, EISSN : 24425613, DOI : -,
Core Subject Science, Education,
Meta Subject Biochemistry, Genetics & Molecular Biology, Chemistry, Mathematics, Physics,
Meta DescJurnal ILMU DASAR (JID) adalah jurnal ilmiah yang melingkupi bidang matematika, fisika, kimia dan biologi. Naskah yang diusulkan untuk diterbitkan Jurnal Ilmu Dasar adalah naskah yang belum pernah diterbitkan dan atau tidak sedang dipertimbangkan penerbitannya di majalah lain. Jurnal Ilmu Dasar menerima tulisan hasil penelitian dasar (bukan aplikasi) untuk bidang matematika, kimia, fisika, dan biologi. Naskah ilmiah ditulis dalam bahasa Indonesia atau dalam bahasa Inggris yang baik. Secara lebih detail dapat dilihat dari pedoman penulisan.
PenulisRatnawati, A. A. Istri , Handayani, Wuryanti , Puspaningsih, Ni Nyoman Tri
Publisher ArticleFakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember
Subtitle Article Jurnal ILMU DASAR Vol 8 No 2 (2007)
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View Articlehttps://jurnal.unej.ac.id/inde…
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