Inulinase has a capacity to convert inulin into fructose hence it could be used in high fructose syrup production. Inulinase is not readily available in Indonesian market. This enzyme could be obtained from thermotolerant yeast found in Dahlia variabilis. Therefore this study was conducted to identify inulinolytic yeast in D. variabilis. The results showed  that an inulinolytic yeast isolate 23 DUCC from D. variabilis tubers was found. The inulinolitic yeast produces inulinase (E.C.3.2.1.7). Based on the identification and determination, the isolate is known to be Kluyveromyces marxianus.  The best optimation condition for this yeast was as follows: concentration of carbon source (inulin) was 0.75%, pH was 5.5 and time of fermentation was 53 hours. In such condition K. marxianus  produced inulinase up to 0.6481 IU.

Published by Universitas Jenderal Soedirman
Journal Name Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
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Location Kab. banyumas, Jawa tengah INDONESIA
Website biosfera| https://journal.bio.unsoed.ac.id/index.php/biosfera|
ISSN ISSN : 08531629, EISSN : 08531629, DOI : -,
Core Subject Science, Agriculture, Social,
Meta Subject Agriculture, Biological Sciences & Forestry, Biochemistry, Genetics & Molecular Biology, Environmental Science,
Meta DescMajalah Ilmiah Biologi Biosfera : A Scientific Journal merupakan peer reviewed jurnal yang diterbitkan oleh Fakultas Biologi Universitas Jenderal Soedirman. Media ini mewadahi hasil-hasil penelitian di bidang biologi tropika yang terbit tiga kali setahun (Januari, Mei, September).
PenulisWijanarka, Wijanarka , Endang, K , Hermin, Hermin
Publisher ArticleFakultas Biologi | Universitas Jenderal Soedirman
Subtitle Article Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 26, No 3 (2009)
Scholar Googlehttp://scholar.google.com/scholar?q=%2Bintitle%3A&…
View Articlehttps://journal.bio.unsoed.ac.…
DOIhttps://doi.org/10.20884/1.mib.2009…
DOI Number DOI: 10.20884/1.mib.2009.26.3.159
Download Article [1] https://journal.bio.unsoed.ac.id/index.p…
Download Article [2] http://download.garuda.ristekdikti.go.id…

 

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