The aim of study was, to identify Clinostomum complanatum (Digenea: Clinostomidae) infectingfreshwater fish in Yogyakarta and Riau on the bases of their molecular profiles in the internal transcribedspacer region (ITS1). Samples of climbing gouramy (Anabas testudineus) infected by Clinostomum sp. wereobtained from Kali Progo River, Yogyakarta. Whereas the climbing perch (Trichogaster trichopterus) wereobtained from the Sail River, Riau. Metacercariae of Clinostomum sp. found in the gills and visceralorgans were aseptically removed using needle, preserved in absolute ethanol. Molecular examination wasperformed by Polymerase Chain Reaction method consisted of extraction, amplification, electrophoresisand sequencing of DNA sample. The DNA sewuences of the samples were analysed by maximum parsimonyand neighbour-joining method. Phylogenetic analysis showed that Clinostomum sp. from Yogyakarta wasgenetically idential to Clinostomum complanatum, whereas Clinostomum sp. from Riau was geneticallysuspected as a new species (difference > 2%) which is included in one cluster to Clinostomum phalacrocorasis.
Published by | Universitas Udayana |
Journal Name | Jurnal Veteriner |
Contact Phone | - |
Contact Name | - |
Contact Email | - |
Location | Kab. badung, Bali INDONESIA |
Website | jvet| https://ojs.unud.ac.id/index.php/jvet| |
ISSN | ISSN : -, EISSN : -, DOI : -, |
Core Subject | Health, |
Meta Subject | Veterinary, |
Meta Desc | Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki |
Penulis | Riauwaty, Morina , ., Kurniasih , Prastowo, Joko , ., Windarti |
Publisher Article | Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association |
Subtitle Article | Jurnal Veteriner Vol 13 No 3 (2012) |
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View Article | https://ojs.unud.ac.id/index.p… |
DOI | http://download.garuda.ristekdikti.… |
DOI Number | Full PDF (307.356 KB) |
Download Article [1] | https://ojs.unud.ac.id/index.php/jvet/ar… |
Download Article [2] | http://download.garuda.ristekdikti.go.id… |
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