Beta-galactoside alpha-2,6-sialyltransferase

beta-galactoside alpha-2,6-sialyltransferase
Identifiers
EC no.	2.4.99.1
CAS no.	9075-81-4
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

In enzymology, a beta-galactoside alpha-2,6-sialyltransferase (EC 2.4.99.1) is an enzyme that catalyzes the chemical reaction

CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine ${\displaystyle \rightleftharpoons }$ CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D- glucosamine

Thus, the two substrates of this enzyme are CMP-N-acetylneuraminate and beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine, whereas its three products are CMP, alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-, and glucosamine.

This enzyme belongs to the family of transferases, specifically those glycosyltransferases that do not transfer hexosyl or pentosyl groups. The systematic name of this enzyme class is CMP-N-acetylneuraminate:beta-D-galactosyl-1,4-N-acetyl-beta-D-glucos amine alpha-2,6-N-acetylneuraminyltransferase. This enzyme participates in n-glycan biosynthesis and glycan structures - biosynthesis 1.

References

• Bartholomew BA, Jourdian GW, Roseman S (1973). "The sialic acids. XV. Transfer of sialic acid to glycoproteins by a sialyltransferase from colostrum". J. Biol. Chem. 248 (16): 5751–62. doi:10.1016/S0021-9258(19)43568-0. PMID 4723915.
• Hickman J, Ashwell G, Morell AG, van den Hamer CJ, Scheinberg IH (1970). "Physical and chemical studies on ceruloplasmin. 8. Preparation of N-acetylneuraminic acid-1-14C-labeled ceruloplasmin". J. Biol. Chem. 245 (4): 759–66. doi:10.1016/S0021-9258(18)63329-0. PMID 4313609.
• Paulson JC, Beranek WE, Hill RL (1977). "Purification of a sialyltransferase from bovine colostrum by affinity chromatography on CDP-agarose". J. Biol. Chem. 252 (7): 2356–62. doi:10.1016/S0021-9258(17)40562-X. PMID 849932.
• Schachter H, Narasimhan S, Gleeson P, Vella G (1983). Glycosyltransferases involved in elongation of N-glycosidically linked oligosaccharides of the complex or N-acetyllactosamine type. Methods Enzymol. Vol. 98. pp. 98–134. doi:10.1016/0076-6879(83)98143-0. ISBN 978-0-12-181998-9. PMID 6366476.
• Spiro MJ, Spiro RG (1968). "Glycoprotein biosynthesis: studies on thyroglobulin. Thyroid sialyltransferase". J. Biol. Chem. 243 (24): 6520–8. doi:10.1016/S0021-9258(18)97805-1. PMID 5726897.