The 2000–01 Toto Cup Al was the 17th season of the third most important football tournament in Israel since its introduction. This was the second edition to be played with clubs of both Israeli Premier League and Liga Leumit clubs.
The competition began on 4 August 2000 and ended on 23 January 2001, with Hapoel Haifa beating Beitar Jerusalem 1–0 in the final.