The 2000–01 Luxembourg Cup was the 76th season of Luxembourg's annual cup competition. It began on September 2, 2000 with Round 1 and finished on May 24, 2001.
Round 1
Fifty-two teams from Division 2 (IV) and Division 3 (V) entered in this round. Forty of them competed in matches, with the other twelve teams were awarded a bye. The games were played on September 3, 2008.
Bye:US Sandweiler, FC UNA Strassen, FC Yellow Boys Weiler-la-Tour, FC Egalité Weimerskirch, AS Colmarberg, FC Marisca Mersch, FC Etoile Sportive Schouweiler, CS Bourscheid.