Analysis of genetic variance, heritability and coefficient of genetic variance were carried out for seven characters of soybean mutant lines in M3 generation, which were conducted at CAIR-Field Station, Pasar Jumat, Jakarta.Results showed that the highest of heritability value about 85.50% was obtained for seed weight, and followed by number of pods,biomass of stem and shelpedpods, root/stem ratio, seed weight/ Zpods ratio, and the lowest heritability value was biomass of root around 26,83% Analysis of coefficient of genetic variance from seven characters of soybean mutants indicated value ranging from 10.00-39,50%.From the result of experiment can be concluded that gamma irradiation produced high genetic variability in M3 generation and contributed positively for crops improvement through breeding programme