French chemist Louis Pasteur was the first 'zymologist' when in 1857 he connected yeast to fermentation. Pasteur originally defined fermentation as "respiration without air".
Pasteur performed careful research and concluded:
Je pense que la fermentation alcoolique ne se produit jamais sans une organization simultanée, une développement, une multiplication de cellules … . Si l'on me demandai en quoi consiste la réaction chimique par laquelle le sucre et décomposé … je l'ignore complètement.
I am of the opinion that alcoholic fermentation never occurs without simultaneous organization, development and multiplication of cells … . If asked, in what consists the chemical act whereby the sugar is decomposed … I am completely ignorant of it.
The GermanEduard Buchner, winner of the 1907 Nobel Prize in chemistry, later determined that fermentation was actually caused by a yeast secretion, which he termed 'zymase'.
The research efforts undertaken by the DanishCarlsberg scientists greatly accelerated understanding of yeast and brewing. The Carlsberg scientists are generally acknowledged[by whom?] as having jump-started the entire field of molecular biology.