Transposon tagging

In genetic engineering, transposon tagging is a process where transposons (transposable elements) are amplified inside a biological cell by a tagging technique. Transposon tagging has been used with several species to isolate genes.[1]^[self-published source?] Even without knowing the nature of the specific genes, the process can still be used.[1]

In plants

By molecular separation of transposons, from a cell nucleus, the cloning is enabled for genes which contain the transposons.[2]^[self-published source?]

By using transposon tagging, researchers have been able to add genetic elements from maize (corn)[3] and Antirrhinum into some other species (such as tobacco,[4] aspen[5] and others).[2] A gene responsible for a particular phenotype can be cloned within a given species, when movement is accompanied by the presence of a mutant phenotype.[2]

References

1. Shah, Paaras V. (2007). "Transposable Elements".
2. McClean, Phillip (1998). "Transposon Tagging".
3. Brutnell, Thomas (2002). "Transposon tagging in maize". Functional & Integrative Genomics. 2 (1–2): 4–12. doi:10.1007/s10142-001-0044-0. PMID 12021846. S2CID 23266030.
4. Dinesh-Kumar, S. P.; Whitham, S.; Choi, D.; Hehl, R.; Corr, C.; Baker, B. (1995). "Transposon Tagging of Tobacco Mosaic Virus Resistance Gene N: Its Possible Role in the TMV-N-Mediated Signal Transduction Pathway". Proceedings of the National Academy of Sciences of the United States of America. 92 (10): 4175–4180. Bibcode:1995PNAS...92.4175D. doi:10.1073/pnas.92.10.4175. JSTOR 2367289. PMC 41906. PMID 7753780.
5. Fladung, M; Deutsch, F; Hönicka, H; Kumar, S (2004). "T-DNA and Transposon Tagging in Aspen". Plant Biology. 6 (1): 5–11. doi:10.1055/s-2003-44745. PMID 15095129. S2CID 260252516.

Further reading

• Transposon Tagging, North Dakota State University
• A.M. Settles, Transposon Tagging and Reverse Genetics, University of Florida