PIPES

This article is about the biochemical buffer PIPES. For other uses, see Pipe (disambiguation).

PIPES

Chemical structure of PIPES
Names
Preferred IUPAC name 2,2′-(Piperazine-1,4-diyl)di(ethane-1-sulfonic acid)
Other names PIPES
Identifiers
CAS Number	5625-37-6^Y
3D model (JSmol)	Interactive image
ChemSpider	72022^N
ECHA InfoCard	100.024.598
PubChem CID	6992709
UNII	G502H79V6L^Y
CompTox Dashboard (EPA)	DTXSID8063965
InChI InChI=1S/C8H18N2O6S2/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16/h1-8H2,(H,11,12,13)(H,14,15,16)^N Key: IHPYMWDTONKSCO-UHFFFAOYSA-N^N InChI=1/C8H18N2O6S2/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16/h1-8H2,(H,11,12,13)(H,14,15,16) Key: IHPYMWDTONKSCO-UHFFFAOYAG
SMILES C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O
Properties
Chemical formula	C8H18N2O6S2
Molar mass	302.37
Appearance	White powder
Melting point	Decomposes above 300 °C
Boiling point	Decomposes
Solubility in water	1 g/L (100 °C)
Hazards
Occupational safety and health (OHS/OSH):
Main hazards	Irritant
NFPA 704 (fire diamond)	1 0 0
Safety data sheet (SDS)	External MSDS
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). N verify (what is ^YN ?) Infobox references

PIPES (piperazine-N,N′-bis(2-ethanesulfonic acid)) is a frequently used buffering agent in biochemistry. It is an ethanesulfonic acid buffer developed by Good et al. in the 1960s.[1]

Applications

PIPES has two pKa values. One pKa (6.76 at 25 °C) is near the physiological pH which makes it useful in cell culture work. Its effective buffering range is 6.1-7.5 at 25 °C. The second pKa value is at 2.67 with a buffer range of from 1.5-3.5. PIPES has been documented minimizing lipid loss when buffering glutaraldehyde histology in plant and animal tissues.[2][3] Fungal zoospore fixation for fluorescence microscopy and electron microscopy were optimized with a combination of glutaraldehyde and formaldehyde in PIPES buffer.[4] It has a negligible capacity to bind divalent ions.[5]

See also

• MOPS
• HEPES
• MES
• Tris
• Common buffer compounds used in biology
• Good's buffers

References

1. Good, Norman E.; Winget, G. Douglas; Winter, Wilhelmina; Connolly, Thomas N.; Izawa, Seikichi; Singh, Raizada M. M. (1966). "Hydrogen Ion Buffers for Biological Research". Biochemistry. 5 (2): 467–77. doi:10.1021/bi00866a011. PMID 5942950.
2. Salema, R. and Brando, I., J. Submicr. Cytol., 9, 79 (1973).
3. Schiff, R.I. and Gennaro, J.F., Scaning Electron Microsc., 3, 449 (1979).
4. Hardham, A.R. (1985). "Studies on the cell surface of zoospores and cysts of the fungus Phytophthora cinnamomi: The influence of fixation on patterns of lectin binding". Journal of Histochemistry. 33 (2): 110–8. doi:10.1177/33.2.3918095. PMID 3918095.
5. "Hopax Fine Chemicals - Biological buffers and their interactions with metal ions".