MRNA (nucleoside-2'-O-)-methyltransferase

mRNA (nucleoside-2'-O-)-methyltransferase
Identifiers
EC no.	2.1.1.57
CAS no.	61970-02-3
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

In enzymology, a mRNA (nucleoside-2'-O-)-methyltransferase (EC 2.1.1.57) is an enzyme that catalyzes the chemical reaction

S-adenosyl-L-methionine + m₇G(5')pppR-RNA ${\displaystyle \rightleftharpoons }$ S-adenosyl-L-homocysteine + m₇G(5')pppRm-RNA (mRNA containing a 2'-O-methylpurine cap)

Thus, the two substrates of this enzyme are S-adenosyl methionine and m7G(5')pppR-RNA, whereas its two products are S-adenosylhomocysteine and m7G(5')pppRm-RNA (mRNA containing a 2'-O-methylpurine cap).

This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. The systematic name of this enzyme class is S-adenosyl-L-methionine:mRNA (nucleoside-2'-O-)-methyltransferase. Other names in common use include messenger ribonucleate nucleoside 2'-methyltransferase, and messenger RNA (nucleoside-2'-)-methyltransferase.

Structural studies

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 2GA9 and 2GAF.

References

• Barbosa E, Moss B (1978). "mRNA(nucleoside-2'-)-methyltransferase from vaccinia virus Purification and physical properties". J. Biol. Chem. 253 (21): 7692–7. doi:10.1016/S0021-9258(17)34425-3. PMID 701281.
• Barbosa E, Moss B (1978). "mRNA(nucleoside-2'-)-methyltransferase from vaccinia virus Characteristics and substrate specificity". J. Biol. Chem. 253 (21): 7698–702. doi:10.1016/S0021-9258(17)34426-5. PMID 701282.
• Boone RF, Ensinger MJ, Moss B (1977). "Synthesis of mRNA guanylyltransferase and mRNA methyltransferases in cells infected with vaccinia virus". J. Virol. 21 (2): 475–83. doi:10.1128/JVI.21.2.475-483.1977. PMC 353847. PMID 833934.
• Ensinger MJ, Martin SA, Paoletti E, Moss B (1975). "Modification of the 5'-terminus of mRNA by soluble guanylyl and methyl transferases from vaccinia virus". Proc. Natl. Acad. Sci. U.S.A. 72 (7): 2525–9. Bibcode:1975PNAS...72.2525E. doi:10.1073/pnas.72.7.2525. PMC 432801. PMID 1058472.
• Groner Y, Gilboa E, Aviv H (1978). "Methylation and capping of RNA polymerase II primary transcripts by HeLa nuclear homogenates". Biochemistry. 17 (6): 977–82. doi:10.1021/bi00599a005. PMID 629955.