Erythromycin 12 hydroxylase

Erythromycin 12 hydroxylase
Identifiers
EC no.	1.14.13.154
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Erythromycin 12 hydroxylase (EC 1.14.13.154, EryK) is an enzyme with systematic name erythromycin-D,NADPH:oxygen oxidoreductase (12-hydroxylating) .[1][2][3] This enzyme catalyses the following chemical reaction

erythromycin D + NADPH + H^+ + O₂ ${\displaystyle \rightleftharpoons }$ erythromycin C + NADP^+ + H₂O

Erythromycin 12 hydroxylase is responsible for the C-12 hydroxylation of the macrolactone ring.

References

1. Lambalot RH, Cane DE, Aparicio JJ, Katz L (February 1995). "Overproduction and characterization of the erythromycin C-12 hydroxylase, EryK". Biochemistry. 34 (6): 1858–66. doi:10.1021/bi00006a006. PMID 7849045.
2. Savino C, Montemiglio LC, Sciara G, Miele AE, Kendrew SG, Jemth P, Gianni S, Vallone B (October 2009). "Investigating the structural plasticity of a cytochrome P450: three-dimensional structures of P450 EryK and binding to its physiological substrate". The Journal of Biological Chemistry. 284 (42): 29170–9. doi:10.1074/jbc.M109.003590. PMC 2781461. PMID 19625248.
3. Montemiglio LC, Gianni S, Vallone B, Savino C (November 2010). "Azole drugs trap cytochrome P450 EryK in alternative conformational states". Biochemistry. 49 (43): 9199–206. doi:10.1021/bi101062v. PMID 20845962.

External links

• Erythromycin+12+hydroxylase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)