Dinocampus coccinellae paralysis virus

Dinocampus coccinellae paralysis virus
Virus classification
(unranked):	Virus
Realm:	Riboviria
Kingdom:	Orthornavirae
Phylum:	Pisuviricota
Class:	Pisoniviricetes
Order:	Picornavirales
Family:	Iflaviridae
Genus:	Iflavirus
Species:	Dinocampus coccinellae paralysis virus

Dinocampus coccinellae paralysis virus (DcPV) is a single-stranded, positive-sense RNA virus of insects, in the picorna-like virus family Iflaviridae, which was first characterised in 2015. It asymptomatically infects the parasitic braconid wasp, Dinocampus coccinellae, and has been proposed to be associated with the paralytic effect the wasp has on its host, the spotted lady beetle, Coleomegilla maculata, which it turns into a so-called "zombie bodyguard" for its pupa.

Taxonomy

Within the family Iflaviridae, the DcPV genome is most closely related to Venturia canescens picorna-like virus and Nasonia vitripennis virus-1.[1]

Distribution

DcPV has been found in D. coccinellae from Canada, Japan, Poland and the Netherlands.[1]

Structure

DcPV's 10,138 nucleotide linear RNA has a single large open reading frame, predicted to encode a 3007 residue polyprotein with non-structural helicase, protease and RNA-dependent RNA polymerase functions in the C-terminal portion and four structural proteins (VP1–4) in the N-terminal portion. The 5′ non-translated region contains cloverleaf and hairpin structures similar to those present in picornaviruses and other picorna-like viruses. The virus particle is around 27 nm in diameter.[1]

Viral life cycle in D. coccinellae

DcPV has not been detected in D. coccinellae eggs. After hatching, the level of the virus genome increases throughout the larval stages, where active viral replication is thought to occur. Higher levels are found in adult wasps, considered to represent inactive stocks of virus. The virus particles are located within large vesicles in cells lining the female wasp's oviduct, where they are sometimes observed to form crystalline arrays. The virus has not yet been shown to cause any disease in the wasp, and the two might have a symbiotic relationship.[1]

Putative effect of DcPV on C. maculata

D. coccinellae pupa adjacent to a C. maculata lady beetle

The Dinocampus coccinellae wasp parasitises the lady beetle species Coleomegilla maculata. The wasp lays a single egg in the beetle's haemocoel where the larva develops, to emerge approximately 20 days later. The wasp larva then pupates with its cocoon underneath the living body of the beetle host. At this point the lady beetle host, which has previously behaved normally, stops moving, is afflicted with twitches or tremors, and becomes a semi-paralysed guardian of the wasp cocoon[1][2] – often referred to as a "zombie bodyguard"[3][4][5] – until the adult wasp emerges a week later. Around a quarter to a third of lady beetles subsequently recover.[2][4]

The exact cause of this dramatic alteration in the beetle's behaviour is unknown, however there is an observation of neuropathy in the ladybug owing to the neurotropic nature of the virus. There is also observation of the downplaying of genes such as Dicer , Ago2, Tlr7 and PI3K.(Both Dicer and Ago2 are involved in gene expression in the organism).[6]

At the time it occurs the parasite and host are no longer in direct contact, as the wasp pupates externally to the lady beetle. Nolwenn Dheilly and co-workers have suggested that the mechanism involves DcPV;[1] some other experts consider that the evidence for this is incomplete.[3][4] DcPV appears to be transmitted to the lady beetle during wasp larval development, with viral RNA being present in the abdomen and head of parasitised lady beetles, but absent from resistant beetles in which the wasp larvae fail to develop. In beetles that recover from paralysis, the level of virus declines significantly. Virus particles, together with lipid droplets, have been observed in glial cells of the cerebral ganglia in parasitised lady beetles. After the wasp larva emerges from the beetle, signs of neuropathy develop including numerous vacuoles in the beetle glial cells.[1] Dheilly and co-workers speculate that this nervous tissue damage is caused by the beetle's immune response and that it mediates the observed change in behaviour, characterising the virus as a "biological weapon" deployed by the wasp against the beetle.[1][3][7]

See also

Other viruses associated with insect behavioural changes:

• Lymantria dispar multicapsid nuclear polyhedrosis virus
• Polydnavirus

References

1. Dheilly NM, Maure F, Ravallec M, et al. (2015), "Who is the puppet master? Replication of a parasitic wasp-associated virus correlates with host behaviour manipulation", Proceedings of the Royal Society B, vol. 282, no. 1803, p. 20142773, doi:10.1098/rspb.2014.2773, PMC 4345448, PMID 25673681
2. Maure F, Brodeur J, Ponlet N, et al. (December 2011), "The cost of a bodyguard", Biology Letters, 7 (6): 843–6, doi:10.1098/rsbl.2011.0415, PMC 3210670, PMID 21697162, archived from the original on 2014-08-08
3. Anonymous (2015), "Wasp virus turns ladybugs into zombie babysitters", Science, doi:10.1126/science.aaa7844
4. Owen J (10 February 2015), "Wasp zombifies ladybugs using virus as bio-weapon—a first", National Geographic, archived from the original on February 12, 2015
5. Rincon P (23 June 2011), "Ladybird made into 'zombie' bodyguard by parasitic wasp", BBC News, BBC, retrieved 12 February 2015
6. M.Dheilly (22 March 2015), "Who is the puppet master? Replication of a parasitic wasp-associated virus correlates with host behaviour manipulation", Proceedings of the Royal Society B: Biological Sciences, 282 (1803), royalsocietypublishing.org, doi:10.1098/rspb.2014.2773, PMC 4345448, PMID 25673681
7. Sekar S (10 February 2015), "Cooperative control", The Scientist