Endopeptidase C30

Peptidase C30, Coronavirus endopeptidase
Identifikasi
SimbolPeptidase_C30
PfamPF05409
InterProIPR008740
PROSITEPS51442
MEROPSC30
SCOPd1q2wb1
SUPERFAMILYd1q2wb1
SARS coronavirus main proteinase
Oktamer proteinase utama SARS.
Pengidentifikasi
Nomor EC3.4.22.69
Basis data
IntEnztinjauan IntEnz
BRENDAentri BRENDA
ExPASytinjauan NiceZyme
KEGGentri KEGG
MetaCycjalur metabolik
PRIAMprofil
Struktur PDBRCSB PDB PDBe PDBsum

Endopeptidase C30 dalam berbagai coronavirus, sering disebut "3C-like proteinase", merupakan famili enzim yang ditemukan dalam poliprotein Coronavirus dan memotong polyprotein di dua situs pembelahan diri. Endopeptidase C30 adalah sisteina protease di bawah klan PA, klasifikasi MEROPS C30.

Komisi Enzim mencatat keluarga ini sebagai "proteinase utama SARS coronavirus" (Nomor EC: 3.4.22.69). Nama umum "3C" mengacu pada Enterovirus protease 3C.

Fungsi

Enzim ini mengkatalisasi reaksi kimia:[1][2][3]

TSAVLQ-SGFRK-NH2 dan SGVTFQ-!GKFKK adalah dua peptida yang sesuai dengan dua situs pembelahan diri dari proteinase seperti SARS 3C

Enzim ini penting dalam pemrosesan polyprotein SARS coronavirus.

Referensi

  1. ^ Goetz DH, Choe Y, Hansell E, Chen YT, McDowell M, Jonsson CB, Roush WR, McKerrow J, Craik CS (July 2007). "Substrate specificity profiling and identification of a new class of inhibitor for the major protease of the SARS coronavirus". Biochemistry. 46 (30): 8744–52. doi:10.1021/bi0621415. PMID 17605471. 
  2. ^ Fan K, Wei P, Feng Q, Chen S, Huang C, Ma L, Lai B, Pei J, Liu Y, Chen J, Lai L (January 2004). "Biosynthesis, purification, and substrate specificity of severe acute respiratory syndrome coronavirus 3C-like proteinase". The Journal of Biological Chemistry. 279 (3): 1637–42. doi:10.1074/jbc.m310875200. PMID 14561748. 
  3. ^ Akaji K, Konno H, Onozuka M, Makino A, Saito H, Nosaka K (November 2008). "Evaluation of peptide-aldehyde inhibitors using R188I mutant of SARS 3CL protease as a proteolysis-resistant mutant". Bioorganic & Medicinal Chemistry. 16 (21): 9400–8. doi:10.1016/j.bmc.2008.09.057. PMID 18845442. 

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