Human recombinant prethrombin-2 (rhPT-2) was expressed in Escherichia coli. Previous studies reported that the expression of rhPT-2 in E. coli tends to formed inclusion body. This study aims to determine the effect of temperature on the expression of rhPT-2 in E. coli ER2566. This study begins with the isolation of recombinant plasmids, competent cell preparation and transformation of competent cells of E. coli ER2566 strain, and rhPT-2 gene expression with various induction temperatures 12, 18, 22 and 30°C. Characterization results showed that the induction temperature of 22°C was the optimum temperature of rhPT-2 gene expression as a soluble fraction in E. coli ER2566 with IPTG concentration 0.1 mM. These results prove that the temperature affects the expression of rhPT-2 in E. coli ER2566. [EFFECT OF TEMPERATURE ON RECOMBINANT HUMAN PRETROMBIN-2 EXPRESSION IN ESCHERICHIA COLI ER2566] (J. Sains Indon., 42(2): 25-30, 2018)Keywords:Induction Temperature, Soluble Fraction, Prethrombin-2, E. coli ER2566
| Published by | Universitas Negeri Medan |
| Journal Name | JURNAL SAINS INDONESIA |
| Contact Phone | - |
| Contact Name | - |
| Contact Email | - |
| Location | Kota medan, Sumatera utara INDONESIA |
| Website | | https://jurnal.unimed.ac.id/index.php/jsi| |
| ISSN | ISSN : -, EISSN : -, DOI : -, |
| Core Subject | Education, |
| Meta Subject | Education, |
| Meta Desc | |
| Penulis | Silaban, Saronom , Simorangkir, Murniaty , Maksum, Iman Permana , Hasan, Khomaini |
| Publisher Article | Universitas Negeri Medan |
| Subtitle Article | Jurnal Sains Indonesia Vol 42, No 2 (2018): Edisi Juli - Desember |
| Scholar Google | http://scholar.google.com/scholar?q=%2Bintitle%3A&… |
| View Article | https://jurnal.unimed.ac.id/20… |
| DOI | https://doi.org/10.24114/jsi.v42i2.… |
| DOI Number | DOI: 10.24114/jsi.v42i2.12245 |
| Download Article [1] | https://jurnal.unimed.ac.id/2012/index.p… |
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Informasi yang terkait dengan Pengaruh Suhu pada Ekspresi Pretrombin 2 Manusia Rekombinan di Escherichia coli ER2566
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