The aim of this study was to find out protein tyrosine kinase profile in Merino Sheep seminal plasma. This study consist of collecting semen of Merino Sheep containing plasma and spermatozoa, the separation between seminal plasma and spermatozoa, then tyrosine kinase analysis using Sodium Dodecyl Sulphate Polyacrilamide Gel Electrophoresis (SDS-PAGE). Semen was collected by artificial vagina and then centrifugated for 40 minutes at 4000 rpm to separated seminal plasma and spermatozoa. Protein was analyzed using SDS-PAGE, to separate each protein based on their molecular weight. The result showed that there were 13 protein bands in 3 seminal plasma samples with an average 149,63 kDa, 139,7 kDa, 114,97 kDa, 109,3 kDa, 97,33 kDa, 93,83 kDa, 86,23 kDa, 77,6 kDa, 64,6 kDa, 52,3 kDa, 41,93 kDa, 38,13 kDa, and 34,5 kDa. In conclusion, it is believed that tyrosine kinase located on the sixth band with molecular weight 93,83 kDa.
| Published by | Universitas Airlangga |
| Journal Name | OVOZOA Journal of Animal Reproduction |
| Contact Phone | +62315992377 |
| Contact Name | Prof. Dr. Imam Mustofa, drh., M.Kes |
| Contact Email | imam.mustofa@fkh.unair.ac.id |
| Location | Kota surabaya, Jawa timur INDONESIA |
| Website | OVZ| https://e-journal.unair.ac.id/OVZ| |
| ISSN | ISSN : 23026464, EISSN : 2722967X, DOI : http://dx.doi.org/10.20473/ovz.v9i1.2020.1-6, |
| Core Subject | Health, Agriculture, |
| Meta Subject | Agriculture, Biological Sciences & Forestry, Veterinary, |
| Meta Desc | Ovozoa Journal of Animal Reproduction (Ovozoa J Anim Reprod) published original articles, review articles, and case reports in Indonesian or English, in the scope of veterinary reproduction and animal reproductive biotechnology from researchers, lecturers, students, and practitioners around Indonesia and worldwide. The main objective of the Ovozoa J Anim Reprod is to disseminate the results of scientific researches in the field of animal reproduction. Ovozoa J Anim Reprod invites manuscripts in the areas: Development of reproductive diagnostic techniques Development of reproductive technologies Infectious and non-infectious reproductive diseases Comparative pathology of reproductive diseases Reproductive endocrinology Infertility Environmental and stress effects on reproduction Better understanding of the in vivo fertilization process Better understanding of embryonic development Artificial insemination and embryo transfer In vitro fertilisation and embryo culture Sexual selection of sperm and embryo Semen and embryo preservation Reproductive cloning Stem cell in reproduction Development of assisted reproductive technologies Molecular biology, genetics, proteomics, metabolomics application to reproductive biology |
| Penulis | Nawangwulan, Tantri , Madyawati, Sri Pantja , Plumeriastuti, Hani , Sardjito, Trilas , Susilowati, Suherni , Safitri, Erma |
| Publisher Article | Universitas Airlangga |
| Subtitle Article | Ovozoa : Journal of Animal Reproduction Vol 7, No 2 (2018): Jurnal Ovozoa : Journal of Animal Reproduction |
| Scholar Google | http://scholar.google.com/scholar?q=%2Bintitle%3A&… |
| View Article | https://e-journal.unair.ac.id/… |
| DOI | https://doi.org/10.20473/ovz.v7i2.2… |
| DOI Number | DOI: 10.20473/ovz.v7i2.2018.91-95 |
| Download Article [1] | https://e-journal.unair.ac.id/OVZ/articl… |
| Download Article [2] | http://download.garuda.ristekdikti.go.id… |
Informasi yang terkait dengan PROFIL PROTEIN TIROSIN KINASE DALAM SEMINAL PLASMA DOMBA MERINO DENGAN TEKNIK SODIUM DODECYL SULPHATE POLYACRILAMIDE GEL ELECTROPHORESIS
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