Cross-breeding between Madura cattle and exotic breeds (exotic cows) occurs in Madura area. The reality in the field shows that the cross-breeding occurs between Limousin cow and Madura cow and the result of the breeding is called as Madrasin cow. One of factor that influences the animal?s growth is Growth Hormone Receptor (GHR). GHR is needed by Growth Hormone (GH) to carry out its effects on target tissues. The purpose of this study is to identify the profile of Growth Hormone Receptors in Madrasin cow. This research was conducted on farmers in Bangkalan, East Java and the Center of Veterinary, Denpasar in MayJune 2017. This study used 14 cows Madrasin. The samples of this study were blood which were taken and used for analyzing DNA that includes DNA isolation, DNA amplification by using Polymerase Chain Reaction (PCR) and Restriction Fragment Length Polymorphism (RFLP) method to determine the genotype. An identification of Growth Hormone Receptor gene polymorphism is done through digesting the DNA fragment of 298 bp by the restriction enzyme AluI. The results shows that the frequency of Growth Hormone Receptor Gene of cow Madrasin allele A was 0.92 and the G allele was 0.08 with frequency of the A allele is higher than G allele.The genotype changing was found in cow Madrasin as much as 0.85 to 0.14 for the AA and AG. As a result of the study, the researcher concluded that Growth Hormone Receptor gene experienced a changing or polymorphisms in Madrasin cow and information which stated in this study be able to used as a basic for selection.
| Published by | Universitas Airlangga |
| Journal Name | OVOZOA Journal of Animal Reproduction |
| Contact Phone | +62315992377 |
| Contact Name | Prof. Dr. Imam Mustofa, drh., M.Kes |
| Contact Email | imam.mustofa@fkh.unair.ac.id |
| Location | Kota surabaya, Jawa timur INDONESIA |
| Website | OVZ| https://e-journal.unair.ac.id/OVZ| |
| ISSN | ISSN : 23026464, EISSN : 2722967X, DOI : http://dx.doi.org/10.20473/ovz.v9i1.2020.1-6, |
| Core Subject | Health, Agriculture, |
| Meta Subject | Agriculture, Biological Sciences & Forestry, Veterinary, |
| Meta Desc | Ovozoa Journal of Animal Reproduction (Ovozoa J Anim Reprod) published original articles, review articles, and case reports in Indonesian or English, in the scope of veterinary reproduction and animal reproductive biotechnology from researchers, lecturers, students, and practitioners around Indonesia and worldwide. The main objective of the Ovozoa J Anim Reprod is to disseminate the results of scientific researches in the field of animal reproduction. Ovozoa J Anim Reprod invites manuscripts in the areas: Development of reproductive diagnostic techniques Development of reproductive technologies Infectious and non-infectious reproductive diseases Comparative pathology of reproductive diseases Reproductive endocrinology Infertility Environmental and stress effects on reproduction Better understanding of the in vivo fertilization process Better understanding of embryonic development Artificial insemination and embryo transfer In vitro fertilisation and embryo culture Sexual selection of sperm and embryo Semen and embryo preservation Reproductive cloning Stem cell in reproduction Development of assisted reproductive technologies Molecular biology, genetics, proteomics, metabolomics application to reproductive biology |
| Penulis | Arganata, Gigih Lesmana , Utomo S, Budi , R.T.S Adikara, R.T.S Adikara |
| Publisher Article | Universitas Airlangga |
| Subtitle Article | Ovozoa : Journal of Animal Reproduction Vol 8, No 1 (2019): Jurnal Ovozoa : Journal of Animal Reproduction |
| Scholar Google | http://scholar.google.com/scholar?q=%2Bintitle%3A&… |
| View Article | https://e-journal.unair.ac.id/… |
| DOI | https://doi.org/10.20473/ovz.v8i1.2… |
| DOI Number | DOI: 10.20473/ovz.v8i1.2019.26-31 |
| Download Article [1] | https://e-journal.unair.ac.id/OVZ/articl… |
| Download Article [2] | http://download.garuda.ristekdikti.go.id… |
Informasi yang terkait dengan PROFIL GEN RECEPTOR GROWTH HORMONE (RGH) PADA SAPI MADRASIN
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