ABSTRACTVanilla is one of the most widely used and important flavoring materials wordwide. The Indonesian natural vanilla has known for having lower quality than Bourbon due to immature harvested bean and uncompleted curing process. The objective of this research was to increase the vanillin content of cured vanilla by improving the ï¢-glucosidase activity. This research studied the best mold (between Aspergillus niger and A. oryzae) and the optimum time for producing ï¢-glucosidase enzyme. The analysis was including the ï¢-glucosidase activity, vanillin and glucovanillin content, and reducing sugar content. The mold that produced the highest ï¢-glukosidase activity was A. niger with 72 incubation time was 193.7 unit/mg protein. The optimum pH and temperature of enzyme from A. niger was 4.5 and 60oC, respectively. The highest vanillin content produced from vanilla homogenat with enzyme extract A. niger at 18 hour incubation time was 0.514 mg/100 ml. The homogenate that is incubated with enzyme extract from A. niger has the highest glucose content at 48 hour incubation time (8.61 mg/ml). The glucovanillin content from three kind of homogenate was decreasing as long as incubation time from 0.44 mg/ml to 0.00 mg/ml (mold), from 0.26 mg/ml to 0.03 mg/ml (emulsin) and from 0.38 mg/ml to 0.12 mg/ml (endogen).Keywords : ï¢-glucosidase activity, vanillin, vanilla
| Published by | Institut Pertanian Bogor |
| Journal Name | Jurnal Teknologi Industri Pertanian |
| Contact Phone | - |
| Contact Name | - |
| Contact Email | - |
| Location | Kota bogor, Jawa barat INDONESIA |
| Website | jurnaltin| http://journal.ipb.ac.id/index.php/jurnaltin| |
| ISSN | ISSN : 02163160, EISSN : 22523901, DOI : 10.24961/j.tek.ind.pert, |
| Core Subject | Agriculture, Engineering, |
| Meta Subject | Agriculture, Biological Sciences & Forestry, Engineering, Industrial & Manufacturing Engineering, |
| Meta Desc | The development of science and technology in agriculture, has been instrumental in increasing the production of various agricultural commodities. But climate change is also uncertain world led to decreased agricultural productivity. World energy crisis resulted in higher prices of agricultural commodities due to competition between food and energy are higher. Efforts to utilize biomass (agricultural products) has started a lot of research, not only as raw material for bioenergy, but also in an attempt to exploit agro-products into valuable economic products, and realize clean production in agro-industry. |
| Penulis | A. Apriyantono, Dwi Setyaningsih, Kania Tresnawati, Maggy T. Soehartono dan |
| Publisher Article | Department of Agroindustrial Technology, Bogor Agricultural University |
| Subtitle Article | Jurnal Teknologi Industri Pertanian Vol. 16 No. 1 (2006): Jurnal Teknologi Industri Pertanian |
| Scholar Google | http://scholar.google.com/scholar?q=%2Bintitle%3A&… |
| View Article | http://journal.ipb.ac.id/index… |
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| Download Article [1] | http://journal.ipb.ac.id/index.php/jurna… |
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Informasi yang terkait dengan PENGARUH AKTIVITAS ï¢ GLUKOSIDASE EKTERNAL DARI KAPANG TERHADAP KADAR VANILIN BUAH VANILI
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