The Mx gene is an antiviral gene used to determine the resistance or the susceptibility to different types of viruses, including the Avian Influenza (AI) virus subtype H5N1. The AI virus subtype H5N1 infection in chickens causes Mx gene polymorphism. The Mx+ gene shows resistant to the AI virus subtype H5N1, whereas the Mx- gene shows signs of susceptible. The objective of this research was to detect the Mx gene in wild aquatic birds using the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method with the primer pairs F2 and NE-R2/R and the RsaI restriction enzyme. DNA samples were obtained from eight species of wild waterbirds with positive and negative exposure to the AI virus subtype H5N1. DNA amplification results showed that the Mx gene in wild aquatic birds is found in a 100 bp fragment, which is the same as the Mx gene found in chickens. However, unlike chickens, the Mx gene in wild aquatic birds did not show any polymorphism. This study proves that Mx- based resistance to AI virus subtype H5N1 in different in wild birds than in chickens. Keywords: Mx gene, wild waterbirds, exposure, AI virus subtype H5N1, resistance Keywords: Mx gene, wild waterbirds, exposure, AI virus subtype H5N1, resistance
| Published by | Universitas Indonesia |
| Journal Name | MAKARA of Science Series |
| Contact Phone | - |
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| Location | Kota depok, Jawa barat INDONESIA |
| Website | science| http://journal.ui.ac.id/index.php/science| |
| ISSN | ISSN : 23391995, EISSN : 23560851, DOI : 10.7454, |
| Core Subject | Science, |
| Meta Subject | Biochemistry, Genetics & Molecular Biology, Chemistry, Earth & Planetary Sciences, Materials Science & Nanotechnology, Physics, |
| Meta Desc | MAKARA Journal of Science publishes high quality research on fundamental science and provides a broad-based forum for the information sharing of ongoing research and development in science. Topics covered include physics, chemistry and biology. All submitted manuscripts will be subjected to the peer review process following a thorough inspection by the editors. Makara Journal of Science is the successor to the "MAKARA of Science Series" and "Makara Sains", which have been published since 1997. Starting from 2014, the journal has been periodically published four times a year (March, June, September, and December). |
| Penulis | Dewi Elfidasari , Dedy Duryadi Solihin , Retno Damayanti Soejoedono , Sri Murtini |
| Publisher Article | Directorate of Research and Community Engagement, Universitas Indonesia |
| Subtitle Article | Makara Journal of Science Vol 17, No 1 (2013): April |
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