The efficacy of Bacillus Calmette-Guerin (BCG), vaccine against tuberculosis (TB), varies widely, from 0 to 90%; and BCG mainly activates CD4+ T cells, but it fails to activate CD8+ T cells. From the previous study, 38-kDa protein is an adhesin protein. CD8+ T cells play the role in controlling Mycobacterium tuberculosis (M.tb) infection and contribute to the memory immunity. The objective of this study was to determine effect of oral immunization by 38-kDa adhesin protein of M.tb to increase the level of CD8+ T cells in the lung of BALB/c mice. This study used an experimental with post test control group design. The mice were divided into six groups (each group consist of 4 samples), where Group 1: were immunization orally with 100 ?g 38-kDa adhesin protein of M.tb and 12 ?g ISCOMs. Followed by group 2: 100 ?g 38-kDa adhesin protein of M.tb, group 3: 50 ?g 38-kDa adhesin protein of M.tb and 12 ?g ISCOMs, and group 4: 50 ?g 38-kDa adhesin protein of M.tb. Group 5: 12 ?g ISCOMs. Group 6: Control. In this study was found increased level of CD8+ T cells in the lung of BALB/c mice after orally immunization with 38-kDa adhesin protein of M.tb. The highest level of CD8+ T cells was on group 1, p=0.000. Also there were found significant differences among the immunized groups, except group 2 and 3, as well as group 5 and 6 also. It can be concluded in this study that oral immunization with 38-kDa adhesin protein of M.tuberculosis could increase the level of CD8+ T cells in the lung of BALB/c mice.
| Published by | Perhimpunan Dokter Spesialis Patologi Klinik Indonesia |
| Journal Name | Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) |
| Contact Phone | +6285733220600 |
| Contact Name | Dr. dr. Puspa Wardhani, SpPK |
| Contact Email | admin@indonesianjournalofclinicalpathology.org |
| Location | Kota adm. jakarta selatan, Dki jakarta INDONESIA |
| Website | patologi| https://www.indonesianjournalofclinicalpathology.org/index.php/patologi| |
| ISSN | ISSN : 08544263, EISSN : 24774685, DOI : -, |
| Core Subject | Health, Science, |
| Meta Subject | Biochemistry, Genetics & Molecular Biology, Health Professions, Medicine & Pharmacology, Neuroscience, |
| Meta Desc | Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) is a journal published by “Association of Clinical Pathologist” professional association. This journal displays articles in the Clinical Pathology and Medical Laboratory scope. Clinical Pathology has a couple of subdivisions, namely: Clinical Chemistry, Hematology, Immunology and Serology, Microbiology and Infectious Disease, Hepatology, Cardiovascular, Endocrinology, Blood Transfusion, Nephrology, and Molecular Biology. Scientific articles of these topics, mainly emphasize on the laboratory examinations, pathophysiology, and pathogenesis in a disease. |
| Penulis | Arthamin, Maimun Z , Gani, Agus A , Issiyah, Nurani , Santoso, Sanarto |
| Publisher Article | Indonesian Association of Clinical Pathologist and Medical laboratory |
| Subtitle Article | INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 18, No 3 (2012) |
| Scholar Google | http://scholar.google.com/scholar?q=%2Bintitle%3A&… |
| View Article | https://www.indonesianjournalo… |
| DOI | https://doi.org/10.24293/ijcpml.v18… |
| DOI Number | DOI: 10.24293/ijcpml.v18i3.377 |
| Download Article [1] | https://www.indonesianjournalofclinicalp… |
| Download Article [2] |
Informasi yang terkait dengan IMUNISASI PROTEIN ADHESIN 38 KDA MYCOBACTERIUM TUBERCULOSIS LEWAT RONGGA MULUT TERKAIT SEL T CD8+ DI PARU
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