The influence of bentonite modification by tetramethyl ammonium hydroxide (TMAH) on its capability to immobilize glucose oxidase (GOX) was studied. Modification of bentonite was conducted by the adding of 0-5% (v/v) TMAH. The observed results show that the different concentrations of TMAH affect the percentage of immobilized enzyme. The results of this study show that the best concentration of TMAH is 5% (v/v) which can immobilize up to 84.71% of GOX. X-ray diffraction (XRD) and Fourier Transforms Infrared Spectroscopy (FTIR) studies have been carried out to observe the structural changes in bentonite due to TMAH modification. The obtained immobilized GOX show the optimum catalytic activity on reaction temperature of 40-50 °C and pH of 7. The immobilized GOX kinetics at the optimum conditions determined the Km and Vmax value to be 4.96x10-2 mM and 4.99x10-3 mM.min-1 respectively. In addition, the immobilized GOX on TMAH-modified bentonite is stable enough so it could be re-used six times before its activity decreased by 39.44%.
| Published by | Universitas Gadjah Mada |
| Journal Name | Indonesian Journal of Chemistry |
| Contact Phone | - |
| Contact Name | - |
| Contact Email | - |
| Location | Kab. sleman, Daerah istimewa yogyakarta INDONESIA |
| Website | ijc| https://journal.ugm.ac.id/index.php/ijc| |
| ISSN | ISSN : 14119420, EISSN : 24601578, DOI : -, |
| Core Subject | Science, Engineering, |
| Meta Subject | Chemical Engineering, Chemistry & Bioengineering, Chemistry, |
| Meta Desc | Indonesian Journal of Chemistry is an International, peer-reviewed, open access journal that publishes original research articles, review articles, as well as short communication in all areas of chemistry including applied chemistry. The journal is accredited by The Ministry of Research, Technology and Higher Education (RISTEKDIKTI) No : 21/E/KPT/2018 (in First Rank) and indexed in Scopus since 2012. Since 2018 (Volume 18), Indonesian Journal of Chemistry publish four issues (numbers) annually (February, May, August and November). |
| Penulis | Chrisnasari, Ruth , Wuisan, Zerlina Gabriela , Budhyantoro, Arief , Widi, Restu Kartiko |
| Publisher Article | Universitas Gadjah Mada |
| Subtitle Article | Indonesian Journal of Chemistry Vol 15, No 1 (2015) |
| Scholar Google | http://scholar.google.com/scholar?q=%2Bintitle%3A&… |
| View Article | http://journal.ugm.ac.id/ijc/a… |
| DOI | https://doi.org/10.22146/ijc.21219 |
| DOI Number | DOI: 10.22146/ijc.21219 |
| Download Article [1] | http://journal.ugm.ac.id/ijc/article/dow… |
| Download Article [2] | http://download.garuda.ristekdikti.go.id… |