The objectives of study were to identify and assess the amino acid diversity of Cytochrome b (Cyt b) gene, genetic marker and characteristic of specific amino acid in Kacang and Ettawa Grade goat. Nineteen heads of Kacang goat (KG) and twelve heads of Ettawa Grade goat (EG) were purposively sampled. The genomic DNA was isolated by Genomic DNA Mini Kit (Geneaid) and amplified Cyt b using PCR method with CytbCapF and CytbCapR primers and was sequenced. The results showed that there were two specific amino acids that distinguish KG and EG goat with C. hircus and C. aegagrus and four specific amino acids that distinguish KG and EG goat with C. falconeri, but there were no specific amino acids can be used as a genetic marker to distinguish between Kacang and EG goat. In conclusion, specific amino acids in Cyt b gene can be used as a genetic marker among KG and EG goat with 3 goat others comparator.
| Published by | Universitas Diponegoro |
| Journal Name | Journal of the Indonesian Tropical Animal Agriculture |
| Contact Phone | - |
| Contact Name | Edy Kurnianto |
| Contact Email | kurniantoedy17@gmail.com |
| Location | Kota semarang, Jawa tengah INDONESIA |
| Website | jitaa| https://ejournal.undip.ac.id/index.php/jitaa| |
| ISSN | ISSN : 20878273, EISSN : 24606278, DOI : -, |
| Core Subject | Science, Agriculture, |
| Meta Subject | Agriculture, Biological Sciences & Forestry, Earth & Planetary Sciences, |
| Meta Desc | Journal of the Indonesian Tropical Animal Agriculture (JITAA) is a double blind peer-reviewed publication devoted to disseminate all information contributing to the understanding and development of animal agriculture in the tropics by publication of original research papers |
| Penulis | Lestari, D. A. , Sutopo, S. , Kurnianto, E. |
| Publisher Article | Diponegoro University |
| Subtitle Article | Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 3 (2017): September |
| Scholar Google | http://scholar.google.com/scholar?q=%2Bintitle%3A&… |
| View Article | https://ejournal.undip.ac.id/i… |
| DOI | https://doi.org/10.14710/jitaa.42.3… |
| DOI Number | DOI: 10.14710/jitaa.42.3.135-146 |
| Download Article [1] | https://ejournal.undip.ac.id/index.php/j… |
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Informasi yang terkait dengan Amino acid diversity on the basis of cytochrome b gene in Kacang and Ettawa Grade goats
Amina Asam amino Klad pengakumulasi asam amino non-protein Sintesis asam amino Sintesis asam amino Strecker Amino Gondohutomo Asam glutamat