Fusarium incarnatum is a fungal pathogen in the genus Fusarium, family Nectriaceae. It is usually associated with over 40 phylogenetic species in the natural environment to form the Fusarium incarnatum-equiseti species complex (FIESC).[1] This complex is widespread across the globe in subtropical and temperate regions,[2] resulting in many reported cases of crop diseases. It produces various mycotoxins including trichothecenes zearalenone,[3] causing both plant and animal diseases.
Taxonomy
Throughout the 20th century, many scientists tried to develop a taxonomy and describe species for Fusarium, but they did not achieve global agreement. Starting from the 1990s, DNA evidence helped introduce new Fusarium species, leading to the publication by Leslie and Summerell in 2006, describing 70 species in the genus. Despite continuous efforts, identifying Fusarium incarnatum remained extremely difficult because of its tight association with other fungal and bacterial species.[4]
Description
Under laboratory conditions, Fusarium incarnatum form a cotton-like fungal colony on potato dextrose agar(PDA).[5] It develops intercalary chlamydospore in its hypha, as well as conidiophore and polyphialide-producing macroconidium. The macroconidia has a curved shape with 3-5 septate, with curved and tapering apical cells and foot-shaped basal cells. FIESC usually has a morphologically similar colony to Fusarium incarnatum, with slightly different colors and textures depending on the species in the complex.[6]
Habitat and distribution
Fusarium incarnatum is widely distributed in tropical and temporal regions. Cases have been reported in China,[7] Brazil,[8] and the United States.[9] With different combinations of fungal and bacteria species involved in FIESC, Fusarium incarnatum can be found in soil, plants, and animals, including humans. Its distribution is most tightly related to crop-growing areas. Some common crops Fusarium incarnatum resides in are wheat, rice, barley, and maize.[10]
Fusarium incarnatum-equiseti species complex
The Fusarium incarnatum-equiseti species complex is a species-rich complex formed by the association of more than 40 possible fungi species. They can be distinguished by identifying their secondary metabolites(SM). During the evolutionary processes of FIESC, the portion of the genome contributing to the biosynthesis of SMs remained similar, but the ability to produce SMs is affected by the distribution of gene clusters.[11]
Pathogen
Fusarium incarnatum is a widespread fungal pathogen that severely impacts crop yield in many places in the world. It can also infect animals and humans and is the cause of several diseases. Cases of Fusarium head blight in wheat caused by FIESC have been reported in Mexico.[12] In humans, an implanted polytetrafluoroethylene dialysis graft with Fusarium incarnatum/equiseti has been reported.[13]
The main toxicity of Fusarium incarnatum comes from the mycotoxin produced. Studies have shown that TRI5, TRI8, and TRI11 protein sequences might be responsible for differential trichothecene analogue production in FIESCs.[14] Many fungicides are developed to treat Fusarium, but there is also a risk that drug resistance could be introduced. Phenamacril-resistant mutants of Fusarium incarnatum are easily induced with high resistance levels.[15]
^Pramunadipta, S., Widiastuti, A., Wibowo, A., Suga, H., & Priyatmojo, A. (2022). Development of PCR-RFLP Technique for Identify Several Members of Fusarium incarnatum-equiseti Species Complex and Fusarium fujikuroi Species Complex. The plant pathology journal, 38(3), 254–260. doi: 10.5423/PPJ.NT.12.2021.0184
^R.K. Gupta, Food Safety in the 21st Century, 2017
^ Avila CF, Moreira GM, Nicolli CP, Gomes LB, Abreu LM, Pfenning LH, Haidukowski M, Moretti A, Logrieco A, Del Ponte EM. Fusarium incarnatum-equiseti species complex associated with Brazilian rice: Phylogeny, morphology and toxigenic potential. Int J Food Microbiol. 2019 Oct 2;306:108267. doi: 10.1016/j.ijfoodmicro.2019.108267. Epub 2019 Jul 13. PMID 31330453.
^Xia, J. W., Sandoval-Denis, M., Crous, P. W., Zhang, X. G., & Lombard, L. (2019). Numbers to names - restyling the Fusarium incarnatum-equiseti species complex. Persoonia, 43, 186–221. https://doi.org/10.3767/persoonia.2019.43.05
^Song, Minjae & Yun, Hye Young & Kim, Young Ho. (2014). Antagonistic Bacillus species as a biological control of ginseng root rot caused by Fusarium cf. Incarnatum. Journal of ginseng research. 38. 136-45. 10.1016/j.jgr.2013.11.016
^MM Wang, Q Chen, YZ Diao, WJ Duan and L Cai, 2019. Fusarium incarnatum-equiseti complex from China, Persoonia. 2019; 43: 70-89. doi: 10.3767/persoonia.2019.43.03
^Santos ACDS, Trindade JVC, Lima CS, Barbosa RDN, da Costa AF, Tiago PV, de Oliveira NT. Morphology, phylogeny, and sexual stage of Fusarium caatingaense and Fusarium pernambucanum, new species of the Fusarium incarnatum-equiseti species complex associated with insects in Brazil. Mycologia. 2019 Mar-Apr;111(2):244-259. doi: 10.1080/00275514.2019.1573047. Epub 2019 Mar 29. PMID 30924728.
^Wang L, Ge SL, Zhao K, Shiwen H. First report of Fusarium incarnatum causing spikelet rot on rice in China. Plant Dis. 2021 Jan 28. doi: 10.1094/PDIS-12-20-2660-PDN. Epub ahead of print. PMID 33507100.
^Imran M, Khanal S, Zhou XS, Antony-Babu S, Atiq M. First Report of Fusarium Sheath Rot of Rice Caused by Fusarium incarnatum-equiseti Species Complex in the United States. Plant Dis. 2022 Apr 29. doi: 10.1094/PDIS-12-21-2693-PDN. Epub ahead of print. PMID 35486602.
^Lu Y, Qiu J, Wang S, Xu J, Ma G, Shi J, Bao Z. Species Diversity and Toxigenic Potential of Fusarium incarnatum-equiseti Species Complex Isolates from Rice and Soybean in China. Plant Dis. 2021 Sep;105(9):2628-2636. doi: 10.1094/PDIS-09-20-1907-RE. Epub 2021 Oct 24. PMID 33393357.
^Villani, A., Proctor, R. H., Kim, H. S., Brown, D. W., Logrieco, A. F., Amatulli, M. T., Moretti, A., & Susca, A. (2019). Variation in secondary metabolite production potential in the Fusarium incarnatum-equiseti species complex revealed by comparative analysis of 13 genomes. BMC genomics, 20(1), 314. https://doi.org/10.1186/s12864-019-5567-7
^Leyva-Mir SG, García-León E, Camacho-Tapia M, Villasenor-Mir HE, Leyva-Madrigal KY, Mora-Romero GA, Tovar-Pedraza JM. Occurrence of the Fusarium incarnatum-equiseti Species Complex Causing Fusarium Head Blight of Wheat in Mexico. Plant Dis. 2022 Mar 12. doi: 10.1094/PDIS-11-21-2467-PDN. Epub ahead of print. PMID 35285265.
^Riddell, J., 4th, Woodside, K. J., Leavitt, M. A., Newton, D. W., & Punch, J. D. (2010). Fusarium incarnatum/equiseti hemodialysis graft infection. Infectious disease reports, 2(2), e14. https://doi.org/10.4081/idr.2010.e14
^Villafana, R. T., & Rampersad, S. N. (2020). Signatures of TRI5, TRI8 and TRI11 Protein Sequences of Fusarium incarnatum-equiseti Species Complex (FIESC) Indicate Differential Trichothecene Analogue Production. Toxins, 12(6), 386. https://doi.org/10.3390/toxins12060386
^Mao Y, Zhao B, Cao Z, Shen J, Xu S, Wu J, Li T, Wang J, Statsyuk N, Shcherbakova L, Zhou M, Duan Y. Risk assessment and molecular mechanism of Fusarium incarnatum resistance to phenamacril. Pest Manag Sci. 2022 Aug;78(8):3394-3403. doi: 10.1002/ps.6967. Epub 2022 May 25. PMID 35514230.
