This study aims to determine the effect of Turnover Ratio and Asset Turnover Ratio on Profitability (ROI) at PT. HM Sampoerna Tbk Period 2009-2018. The method used is explanatory research with a sample of 85 respondents. The analysis technique uses statistical analysis with regression testing, evaluation, determination and hypothesis testing. The results of this study indicate that a significant Turnover Ratio to Profitability (ROI) of 91.8%, the hypothesis test obtained a significance of 0,000 <0.05. Asset Turnover Ratio has a significant effect on profitability (ROI) of 96.3%, hypothesis testing obtained significance of 0,000 <0.05. Planning Turnover Ratio and Activation Turnover Ratio significantly to Profitability (ROI) of 96.5%, the hypothesis test obtained significance of 0,000 <0.05.