ABSTRACTThe objectives of this study were to analyze incubation period of biofungicide extract to controlDrechslera oryzae pathogen on rice in vitro. The research was conducted from August toNovember , 2016 at Departement Budidaya Pertanian laboratory, used randomized completelydesign, with 35 treatment and 3 replications. Eight of biofungiside were used in this study,there are lerak fruits, siam weed leaves, taya leaves, gelinggang leaves, meniran, roots andtegari stem, galam leaves and 5 incubation periode (0, 1, 2, 3, 4 week) . The results showed that0–4 week incubation periode of lerak fruits extract had clear effective to inhibit D. oryzaecolony.Key words:Biofungicide extract, Drechslera oryzae, incubation period.