Methylosinus trichosporium

Methylosinus trichosporium
Scientific classification
Domain:
Bacteria
Phylum:
Pseudomonadota
Class:
Alphaproteobacteria
Order:
Hyphomicrobiales
Family:
Methylocystaceae
Genus:
Methylosinus
Species:
M. trichosporium
Binomial name
Methylosinus trichosporium
Bowman et al. 1993[1]
Type strain
ACM 3311, ATCC 35070, IMET 10541, NCIMB 11131, OB3b, UNIQEM 75, VKM B-2117[2]

Methylosinus trichosporium is an obligate aerobic and methane-oxidizing bacterium species from the genus of Methylosinus.[1][3][4][5][6] Its native habitat is generally in the soil, but the bacteria has been isolated from fresh water sediments and groundwater as well.[7] Because of this bacterium's ability to oxidize methane, M. trichosporium has been popular for identifying both the structure and function of enzymes involved with methane oxidation since it was first isolated in 1970 by Roger Whittenbury and colleagues.[4][6] Since its discovery, M. trichosporium and its soluble monooxygenase enzyme have been studied in detail to see if the bacterium could help in bioremediation treatments.[8]

Biology

As a type II methanotroph, M. trichosporium relies on methane as its primary source of carbon and energy.[9] A commonly used strain of this bacteria is strain OB3b, which is available through the American Type Culture Collection.[9] Even though all methanotrophs can form particulate methane monooxygenase (pMMO), the ability to produce soluble methane monooxygenase (sMMO) is limited to type II methanotrophs.[10] These enzymes perform the same purpose for cellular function, but sMMO has a much higher specificity compared to pMMO.[8] Another key difference between the sMMO and pMMO is that they are produced under different conditions. In environments with concentrations of copper lower than 0.25 μM, sMMO is produced, but in higher concentrations of copper, sMMO production is lost and pMMO is produced.[9]

Applications in bioremediation

The ability to produce sMMO is of particular interest to researchers due to its ability to degrade trichloroethene (TCE) at a magnitude one order higher than other microbial cultures.[9][8] For example, in one study on removal of TCE from sources at as high of concentration as 50 mg/mL, M. trichosporium was shown to be able to remove up to 99% of TCE.[9] Despite the efficiency of sMMO, the products formed from degrading TCE are toxic to M. trichosporium. This bacterium already grows relatively slowly, so toxic effects of TCE degradation products have made it challenging to use M. trichosporium in bioremediation treatments.[8] The effect of copper on strain OB3b's ability to produce sMMO is also a limiting effect of what environments M. trichosporium can be used in.

References

  1. ^ a b LPSN lpsn.dsmz.de
  2. ^ Straininfo of Methylosinus trichosporium
  3. ^ UniProt
  4. ^ a b Stein, L. Y.; Yoon, S.; Semrau, J. D.; DiSpirito, A. A.; Crombie, A.; Murrell, J. C.; Vuilleumier, S.; Kalyuzhnaya, M. G.; Op den Camp, H. J. M.; Bringel, F.; Bruce, D.; Cheng, J.- F.; Copeland, A.; Goodwin, L.; Han, S.; Hauser, L.; Jetten, M. S. M.; Lajus, A.; Land, M. L.; Lapidus, A.; Lucas, S.; Medigue, C.; Pitluck, S.; Woyke, T.; Zeytun, A.; Klotz, M. G. (15 October 2010). "Genome Sequence of the Obligate Methanotroph Methylosinus trichosporium Strain OB3b". Journal of Bacteriology. 192 (24): 6497–6498. doi:10.1128/JB.01144-10. PMC 3008524. PMID 20952571.
  5. ^ editors, Don J. Brenner, Noel R. Krieg, James T. Staley (2005). Bergey's manual of systematic bacteriology (2nd ed.). New York: Springer. ISBN 978-0-387-29298-4. {{cite book}}: |last1= has generic name (help)CS1 maint: multiple names: authors list (link)
  6. ^ a b Whittenbury, R.; Phillips, K. C.; Wilkinson, J. F.YR 1970 (1970). "Enrichment, Isolation and Some Properties of Methane-utilizing Bacteria". Microbiology. 61 (2): 205–218. doi:10.1099/00221287-61-2-205. ISSN 1465-2080. PMID 5476891.{{cite journal}}: CS1 maint: numeric names: authors list (link)
  7. ^ Knief, Claudia (2015-12-15). "Diversity and Habitat Preferences of Cultivated and Uncultivated Aerobic Methanotrophic Bacteria Evaluated Based on pmoA as Molecular Marker". Frontiers in Microbiology. 6: 1346. doi:10.3389/fmicb.2015.01346. ISSN 1664-302X. PMC 4678205. PMID 26696968.
  8. ^ a b c d Sullivan, Jonathan P.; Dickinson, David; Chase, Howard A. (1998-01-01). "Methanotrophs, Methylosinus trichosporium OB3b, sMMO, and Their Application to Bioremediation". Critical Reviews in Microbiology. 24 (4): 335–373. doi:10.1080/10408419891294217. ISSN 1040-841X. PMID 9887367.
  9. ^ a b c d e Phelps, Patricia A.; Agarwal, Sandeep K.; Speitel, Gerald E.; Georgiou, George (November 1992). "Methylosinus trichosporium OB3b Mutants Having Constitutive Expression of Soluble Methane Monooxygenase in the Presence of High Levels of Copper". Applied and Environmental Microbiology. 58 (11): 3701–3708. Bibcode:1992ApEnM..58.3701P. doi:10.1128/aem.58.11.3701-3708.1992. ISSN 0099-2240. PMC 183163. PMID 16348810.
  10. ^ Rodrigues, Andréa dos Santos; Valdman, Belkis; Salgado, Andréa Medeiros (June 2009). "Analysis of methane biodegradation by Methylosinus trichosporium OB3b". Brazilian Journal of Microbiology. 40 (2): 301–307. doi:10.1590/S1517-83822009000200017. ISSN 1517-8382. PMC 3769742. PMID 24031362.

Further reading

  • Stein, L. Y.; Yoon, S.; Semrau, J. D.; DiSpirito, A. A.; Crombie, A.; Murrell, J. C.; Vuilleumier, S.; Kalyuzhnaya, M. G.; Op den Camp, H. J. M.; Bringel, F.; Bruce, D.; Cheng, J.- F.; Copeland, A.; Goodwin, L.; Han, S.; Hauser, L.; Jetten, M. S. M.; Lajus, A.; Land, M. L.; Lapidus, A.; Lucas, S.; Medigue, C.; Pitluck, S.; Woyke, T.; Zeytun, A.; Klotz, M. G. (15 October 2010). "Genome Sequence of the Obligate Methanotroph Methylosinus trichosporium Strain OB3b". Journal of Bacteriology. 192 (24): 6497–6498. doi:10.1128/JB.01144-10. PMC 3008524. PMID 20952571.
  • Sullivan, JP; Dickinson, D; Chase, HA (1998). "Methanotrophs, Methylosinus trichosporium OB3b, sMMO, and their application to bioremediation". Critical Reviews in Microbiology. 24 (4): 335–73. doi:10.1080/10408419891294217. PMID 9887367.
  • editors, Don J. Brenner, Noel R. Krieg, James T. Staley (2005). Bergey's manual of systematic bacteriology (2nd ed.). New York: Springer. ISBN 978-0-387-29298-4. {{cite book}}: |last1= has generic name (help)CS1 maint: multiple names: authors list (link)
  • Gribble, volume editor, Gordon W. (2003). Natural production of organohalogen compounds. Berlin: Springer. ISBN 978-3-540-45293-5. {{cite book}}: |first1= has generic name (help)CS1 maint: multiple names: authors list (link)
  • al.], edited by Hans F. Stroo, Andrea Leeson, C. Herb Ward ; authors, Wayne R. Amber ... [et (2013). Bioaugmentation for groundwater remediation. New York: Springer. ISBN 978-1-4614-4115-1. {{cite book}}: |first1= has generic name (help)CS1 maint: multiple names: authors list (link)
  • al.], volume editor: A.H. Neilson ; with contributions by A.-S. Allard ... [et (2002). Anthropogenic compounds. Berlin: Springer-Verlag. ISBN 978-3-540-42064-4. {{cite book}}: |first1= has generic name (help)CS1 maint: multiple names: authors list (link)
  • Schlegel, edited by H. G.; Barnea, J. (1977). Microbial Energy Conversion The Proceedings of a Seminar Sponsored by the UN Institute for Training and Research (UNITAR) and the Ministry for Research and Technology of the Federal Republic of Germany Held in Göttingen, October 1976. Burlington: Elsevier Science. ISBN 978-1-4831-3912-8. {{cite book}}: |first1= has generic name (help)


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