On 13 October 2007, the frequency of the route was reduced to compensate for the introduction of new route 332 which commenced operating on the same date.[3]
New Routemaster buses were introduced on 26 September 2015. The rear platform remains closed at all times except when the bus is at bus stops.[4]
In 2021, the frequency of the service was reduced from 8 buses per hour to 6 during Monday-Saturday peak times, and from 6 buses per hour to 5 at other times.[5]
In July 2022, Transport for London opened a public consultation on proposals for the route to be withdrawn as part of the Central London Buses Review.[6] In November 2022, it was announced that the route would not be withdrawn but would instead run between Paddington and Brent Park, fully replacing route 332. This change was implemented on 29 April 2023.[7][8]