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KIAA0895
KIAA0895 is a protein that in Homo sapiens is encoded by the KIAA0895 gene. The gene encodes a protein commonly known as the KIAA0895 protein. Its aliases include hypothetical protein LOC23366, OTTHUMP00000206979, OTTHUMP00000206980, 9530077C05Rik, and 1110003N12Rik.[5] It is located at 7p14.2.[6] Research into the KIAA proteins has shown that they are similar to known genes with functions related to cell signaling/communication, cell structure/motility and nucleic acid management.[7] GeneLocusThe KIAA0895 gene is located at 7p14.2.[6] The genomic DNA is 65,976 base pairs long,[8] while the longest mRNA that it produces is 4463 bases long. It can be transcribed into 15 transcript variants, which in turn can produce 13 different isoforms of the protein.[9]  Gene NeighborhoodKIAA0895 is surrounded by the following genes on chromosome 7:[8] Size of geneThe gene encoded for the KIAA0895 protein is 65,975 nucleotides long, from nucleotides 36324150 to 36390125, with seven exons. mRNAThere are ten different isoforms for KIAA0895.[6]
ProteinThe longest protein isoform that is produced by the KIAA0895 gene is termed LOC23366 isoform 1 and is 520 amino acids long.[10] The predicted molecular weight is 61kDa.[11] Additionally, the theoretical isoelectric point is 10.[11] Amino acid compositionKIAA0895 is a lysine and arginine semi-enriched protein.[12] KIAA0895 is semi-enriched in positively charged lysine and arginine groups, and positively and negatively charged lysine, arginine, glutamic acid and aspartic acid groups.[12] However, KIAA0895 is semi-depleted in non-polar alanine, glycine and proline groups.[12] The charge distribution analysis shows that there are no negative or mixed charge clusters.[12] However, there is one positive charge cluster from amino acids 12 to 36.[12]
RegionsLOC23366 contains a protein domain of unknown function called DUF1704.[13] It also contains a region of low complexity from position 120 to position 150 in the protein,[14] and an arginine-rich area from position 12 to position 51.[15] PromotersUsing ElDorado by Genomatrix, a promoter region sequence was found.[16] The most likely promoter for KIAA0895 starts at 36389926 and goes to 36391149, with a length of 1224.[16]  Post-translational modificationKIAA0895 is predicted to undergo phosphorylation at several serines, threonines, and tyrosines throughout its structure.[17] Phosphorylation at these sites is a form of gene regulation. Phosphorylation results in a conformational change in the structure of many enzymes and receptors. This causes them to become activated or deactivated. Stem loopsUsing a database called Mfold, a stem-loop formation for the 5' UTR region shows that there is a lack of conservation, meaning there is some precedence that these stems and loops are not used for translation regulation.[18] The ΔG value was -12.90 kcal/mol with three loops.[18] Using the same database, a stem-loop formation for the 3' UTR region shows that there is conservation, meaning there is some precedence that they are used for translation regulation.[18] The ΔG value was -636.80 kcal/mol.[18] Tertiary structureKIAA0895 has a tertiary structure with alpha helices and beta sheets.[19]   Interacting proteinsThere are three proteins likely to be interacting proteins with KIAA0895. These proteins are ELAVL1,[20] vata,[21] and glym.[21] These interactions have experimental evidence from the sources provided. ExpressionKIAA0895 is most commonly found in the testis, however it also has a strong expression in the kidneys, adrenal glands, and brain.[6]   Per Health StateUsing an EST profile from NCBI, KIAA0895 has strong expression in cervical tumors and bladder carcinoma.[22]  Interacting proteinsUsing three different databases, three different interacting proteins were found. These include ELAVL1, VATA, and GLYM.[21] There was experimental evidence for all three of these interacting proteins. Homologs and orthologsKIAA0895 has over 228 orthologs.[6] Orthologs have been found in mammals and eukaryotes.[23] There are homologs in 9 species.[6] The full list of organisms in which homologs have been found is given below. ParalogsKIAA0895 has 7 paralogs in Homo sapiens:[23]
References
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