K-16 continues through Valley Falls, crossing the Delaware River until meeting with US-59. K-16 then runs concurrent with US 59 through Oskaloosa until heading east again through McLouth and winding through Leavenworth County until coming to an end at an intersection with US-24 and US-40 in Tonganoxie.
A southern terminus was located in Montgomery County and Neosho County starting in Chanute and ending at the Oklahoma state line in Coffeyville.
In 1932, the southern section was replaced by US 169. In 1935, the remainder of K-16 became part of K-7. K-16 was reassigned as a renumbering of K-24 because of the extension of US-24 into Kansas.