Pielak is the Kenan Distinguished Professor[1] of Chemistry, Biochemistry and Biophysics at the University of North Carolina at Chapel Hill School of Medicine,[2] Lineberger Comprehensive Cancer Center,[3] and Department of Chemistry.[4] He is a Fellow of the Biophysical Society.[5][6]
Using in-cell nuclear magnetic resonance spectroscopy, a technique he helped develop,[12] Pielak determined that the effects of in-cell crowding do not arise solely from the close-packed nature of the cytoplasm but rather that repulsive and attractive chemical interactions between cellular components determine the effects of macromolecular crowding. These interactions organize the inside of cells, controlling metabolism and signaling. He and his collaborators have presented a quantitative model to explain crowding effects that is independent of crowder identity.[13][14][15][16] For his work in this area, he received an NIH Director's Pioneer Award in 2006.[17]
Selected honors
Gary Pielak has been on the Editorial Advisory Board of the journal Protein Science, and serves on the Editorial Board of the journal Magnetic Resonance Letters[18]. He was an invited speaker at the 2017 Nobel Symposium on Protein Folding: From Mechanisms to Impact on Cells, in Stockholm, Sweden.[19]
In 2023, Pielak received both the Johnston Teaching Excellence Award and the Faculty Award for Excellence in Doctoral Mentoring[20] from the University of North Carolina at Chapel Hill. He also presented the McElvain Lecture at the University of Wisconsin, Madison that year.[citation needed] In 2024, he was an invited speaker at Protein Folding Dynamics Gordon Research Conference in Galveston, Texas.[21]
Selected publications
"Physicochemical properties of cells and their effects on intrinsically disordered proteins (IDPs)" (2014). Francois-Xavier Theillet, Andres Binolfi, Tamara Frembgen-Kesner, Karan Hingorani, Mohona Sarkar, Ciara Kyne, Conggang Li, Peter B Crowley, Lila Gierasch, Gary J Pielak, Adrian H Elcock, Anne Gershenson, Philipp Selenko. Chemical Reviews 114 (13), 6661-6714.[22]
"FlgM gains structure in living cells" (2002). Matthew M Dedmon, Chetan N Patel, Gregory B Young, Gary J Pielak, Proceedings of the National Academy of Sciences 99 (20), 12681-12684.[23]
"Impact of Protein Denaturants and Stabilizers on Water Structure" (2004). Joseph D. Batchelor, Alina Olteanu, Ashutosh Tripathy, and Gary J. Pielak, J. Am. Chem. Soc. 2004, 126, 7, 1958–1961.[24]
"Macromolecular Crowding and Protein Stability" (2012). Yaqiang Wang, Mohona Sarkar, Austin E. Smith, Alexander S. Krois, and Gary J. Pielak, J. Am. Chem. Soc. 2012, 134, 40, 16614–16618.[25]
