Kromatografi cair kinerja tinggi

High-performance liquid chromatography
An HPLC. From left to right: A pumping device generating a gradient of two different solvents- a steel-enforced column and a detector for measuring the absorbance.
AkronimHPLC
KlasifikasiChromatography
Analisisorganic molecules
biomolecules
ions
polymers
Other techniques
Terhubung juga denganChromatography
Aqueous normal-phase chromatography
Hydrophilic Interaction Chromatography
Ion exchange chromatography
Size exclusion chromatography
Micellar liquid chromatography
Ditulis dengan tanda hubungLiquid chromatography-mass spectrometry
A modern self-contained HPLC.
Schematic representation of an HPLC unit. (1) Solvent reservoirs, (2) Solvent degasser, (3) Gradient valve, (4) Mixing vessel for delivery of the mobile phase, (5) High-pressure pump, (6) Switching valve in "inject position", (6') Switching valve in "load position", (7) Sample injection loop, (8) Pre-column (guard column), (9) Analytical column, (10) Detector (i.e., IR, UV), (11) Data acquisition, (12) Waste or fraction collector.

Kromatografi cair kinerja tinggi, disingkat KCKT ((Inggris): high performance liquid chromatography, disingkat HPLC), merupakan salah satu teknik kromatografi untuk zat cair yang biasanya disertai dengan tekanan tinggi. Seperti teknik kromatografi pada umumnya, HPLC berupaya untuk memisahkan molekul berdasarkan perbedaan afinitasnya terhadap zat padat tertentu. Cairan yang akan dipisahkan merupakan fase cair dan zat padatnya merupakan fase diam (stasioner). Teknik ini sangat berguna untuk memisahkan beberapa senyawa sekaligus karana setiap senyawa mempunyai afinitas selektif antara fase diam tertentu dan fase gerak tertentu. Dengan bantuan detektor serta integrator kita akan mendapatkan kromatogram. Kromatorgram memuat waktu tambat serta tinggi puncak suatu senyawa.


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